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In agreement with the role of Mlkl in necroptosis, TNF+CHX+zVAD or TNF+SmacM+zVAD induced necroptosis was significantly reduced in Mlkl MEFs compared to wild type MEFs (Figure 3B and 3C), confirming the notion that Mlkl is important for necroptosis, but not for apoptosis. Similarly, while apoptotic cell death induced by TRAIL, another TNF family member, plus CHX was not affected by Mlkl deletion, TRAIL+CHX+zVAD induced necroptosis was compromised considerably in the absence of Mlkl (Figure 3D).
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